The effect of membrane filtration on estimates of microbial ATP in New Zealand freshwaters     

R.D. Pridmore  C.W. Hickey  J. E. Hewitt 《新西兰海洋与淡水研究杂志》2013,47(3-4):311-315

Concentrations of microbial ATP in 15 New Zealand lakes were determined using 2 extraction techniques. ATP was found in both the paniculate (>0.45 μm) and dissolved (<0.45 μm) fractions of the water samples. Higher concentrations of paniculate ATP were obtained when ATP was extracted directly from freshwater samples rather than following pre‐concentration on to membrane filters. Recovery of particulate ATP declined with increasing volumes of sample filtered. The magnitude of the filtration effect varied unpredictably between lakes and within lakes with time. Corrections for the presence of dissolved ATP were necessary before estimates of particulate ATP could be made by the direct extraction technique. Concentrations of dissolved ATP up to 1.2 μg L^‐1 were observed. The direct extraction technique is recommended for more accurate determination of particulate ATP in New Zealand freshwaters.  相似文献   

Letters to the editor     

Malcolm Haddon  Robert C. Francis 《新西兰海洋与淡水研究杂志》2013,47(4):447-449

A preliminary scuba survey of oligotrophic Lake Rotoma in 1972 revealed a vegetation mainly composed of native hydrophytes in which exotics were at an early stage of colonisation. In 1973 the presence of species was recorded in 5708 quadrats (625 cm²) at 1 m intervals along a total of 50 line transects placed systematically around the lake. Water depth was measured, and quadrat cover and substrate type were subjectively estimated. Species frequency calculations showed that the dominant vegetation pattern was a characean meadow of Chara fibrosa f. acanthopitys (A.Br.) R.D.W., Nitella leptostachys var. leonhardii (R.D.W.) R.D.W., and N. pseudoflabellata var. mucosa (Nordst.) Bailey. The charophytes extended over a depth range of 1–17.5 m on a wide variety of substrates and gradients. Native vascular plants were absent from many transects, and had a depth range only from 0 to 4.5 m, with most occurring above 3.5 m. The Low Mixed Community, found in shallow water less than 1.25 m in depth at the northeast end of the lake, provided this area with a high species diversity. Exotic hydrophytes had established in many areas around the lake. The distribution of Lagarosiphon major (Ridley) Moss and Elodea canadensis Michx. appeared to coincide with boating access and fallen submerged trees over a depth range of 0–6.0 m, although much of the available habitat had not yet been exploited. Emergent species were most abundant within the southwest inlet and also in the lagoons surrounding the lake where sheltered conditions and shallow gradients prevail.  相似文献   

Limnology of Opal Lake     

D. J. Forsyth  A. L. MacKenzie 《新西兰海洋与淡水研究杂志》2013,47(3):279-283

Opal Lake, a cold, acid lake on the central volcanic plateau of the North Island, New Zealand, had an average pH of 4.3 and was highly eutrophic. The biota was less diverse than in neighbouring near‐neutral waters. Macrophytes were rare; phytoplankton were mainly represented by the Chlorophyceae, and there were only 4 zooplankton species. The mean standing crop of macroinvertebrates was 6240 per square metre; this was higher than in other eutrophic lakes in the region and comprised more than 98% chironomid larvae represented by 4 species. Molluscs were absent. The fauna was more diverse than in more acid lakes of the .central volcanic plateau.  相似文献   

Influence of the water layer upon the depth of ground radar investigations in freshwater reservoirs     

M. L. Vladov  A. M. Pyatilova 《Moscow University Geology Bulletin》2009,64(1):59-62

A number of problems associated with detailed investigation of subsurface geological structure below the bottoms of freshwater reservoirs (rivers, ponds, and lakes) arise during the study of the upper portions of geological sections. The present study is aimed at marginal estimation of the penetrating depth of ground radar investigations in freshwater reservoirs (based on bottom reflections), taking into account the complex nature of conductivity and its dependence upon the frequency under ideal conditions with no noise. The same method can be applied for estimating any given dynamic recording range.  相似文献   

Geologic and hydrologic settings for development of freshwater lenses in arid lands   总被引：2，自引：0，他引：2       下载免费PDF全文

Adam Milewski  Mohamed Sultan  Ahmad Al‐Dousari  Eugene Yan 《水文研究》2014,28(7):3185-3194

Satellite observations were used to test the validity of previously identified favourable conditions for the formation of freshwater lenses, identify additional potential occurrences, and model modern potential recharge in the Raudhatain Watershed (3696) in northern Kuwait. Favourable conditions include infrequent yet intensive precipitation events, drainage depressions to collect the limited runoff, and presence of conditions (e.g. high infiltration capacity) that promote groundwater recharge and preservation (e.g. underlying saline aquifer) of infiltrating groundwater as freshwater lenses floating over saline aquifer water due to differences in density. Specifically, the following field and satellite‐based observations were noted for the Raudhatain Watershed: (1) Over ~30 precipitation events were identified from the Tropical Rainfall Measuring Mission precipitation data (1998–2009); (2) slope is gentle (2 m/km), and the surface is largely (80%) covered by alluvial deposits with high infiltration capacities (up to 9 m/day); (3) no flows and long‐term ponding were reported at the watershed outlet or detected from Landsat thematic mapper images; (4) infiltration is high based on increases in soil moisture content (from an advanced microwave scanning radiometer) and vegetation index following large precipitation events; and (5) freshwater lenses that overlie highly saline [total dissolved solids (TDS): >35 000] unconfined aquifers underlying the watershed are absent in the southern regions, where infiltrating fresh water mixes with the less saline groundwater (TDS: <10 000). Twenty potential locations (size: 1 to 75 km²) for freshwater lens development were identified in northern Kuwait, and continuous rainfall–runoff models (Soil Water and Assessment Tool) were applied to provide a first‐order estimation of the average annual recharge in the watershed (127 × 10⁶ m³) and freshwater lenses (8.17 × 10⁶ m³). Results demonstrate the settings for enhanced opportunities for groundwater recharge, outline the amounts of and preservation conditions for the groundwater feeding the freshwater lenses, and highlight potential applications and locations of freshwater lenses in similar settings elsewhere in the Arabian Peninsula and beyond. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   

微藻矿化去除Pb2+的研究   总被引：1，自引：0，他引：1       下载免费PDF全文

韩冉  林文挺  杨鹏  黄振  刘明华  程扬健 《高校地质学报》2015,21(4):608

藻类作为水体中的初级生产力,通过生物积累、生物矿化等生理功能与环境中的重金属相互作用、对重金属地球生 物化学循环起到关键作用。为了探讨藻类生物矿化去除水体中重金属的现象,文中研究了淡水微藻FZUL-321对Pb2+的去除 及矿化。结果表明：微藻FZUL-321对Pb2+有较强的去除能力,且是一个快速去除的过程。随着Pb2+浓度增大,该微藻对Pb2+ 的去除效果也增大。在弱酸性条件下（pH5.0）,其去除Pb2+的效果较好。如在Pb2+初始浓度为100mg/L,pH5.0,去除时间 为40min,此时Pb2+的去除量为423.2×10-3干重。原子力显微镜（AFM）对细胞表面的形貌进行观察,发现微藻FZUL-321与 Pb2+作用后,细胞形貌和尺寸变化较大,如细胞褶皱并塌陷,细胞表面变得粗糙等。傅里叶红外光谱（FT-IR）结果表明藻 细胞表面的羧基、氨基和磷酸基团等官能团参与前期Pb2+的吸附沉淀。最终,通过一系列生化作用,微藻FZUL-321将离子 态的Pb2+矿化,X射线衍射（XRD）分析显示,矿化产物为Pb5(PO4)3OH。  相似文献   

Preliminary investigations on Arctic microalgae by joint aDDlication of fluorescent instruments     

ZHANG Fang  HE Jianfeng  GUANG Yingzhi 《极地研究(英文版)》2012,(2):128-132

In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima （Bacillariophyceae） and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state （Fo）, actual photochemical efficiency of PSll （ФPSII）, and electron transport rate （ETR） exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.  相似文献   

Fauna and standing crop of freshwater mussels in Poyang Lake, China     

熊六凤  欧阳珊  吴小平 《中国海洋湖沼学报》2012,30(1):124-135

Freshwater mussels are a major component of the zoobenthos and they therefore play an important role in freshwater ecosystems. The community composition and the standing crop of freshwater mussels in Poyang Lake, the largest freshwater lake in China and one of two lakes connected to the Changjiang (Yangtze) River, were investigated from February 2008 to May 2009. Mussels were sampled in hydrologically and geographically distinct sampling areas. A total of 42 species and 13 genera were identified, of which 32 species were endemic to China. Two species, Unio douglasiae and Lamprotula caveata, dominated all sampling areas. The number of genera and species, and the number of species within each genus, differed significantly among sampling areas. Freshwater mussels were very abundant, with an overall density of 0.28 ± 0.22 ind./m2 and biomass of 4.08 ± 3.96 g/m2. Significant variation observed among sampling areas and among genera was probably due to the variety of habitats among the areas sampled and to the adaptability of different freshwater mussels to environments. This study suggests that Poyang Lake and other freshwater lakes in the region are important sites of freshwater mussel biodiversity and conservation.  相似文献   

外源氮输入对沼泽湿地小叶章枯落物性质及其早期分解的影响   总被引：6，自引：0，他引：6  

刘德燕  宋长春 《湿地科学》2008,6(2)

在三江平原沼泽湿地,选取典型湿地植物小叶章(Calamagrostis angustifolia)为研究对象,对比研究了连续施氮[24g/(m2·a)]3a的小叶章立枯物(N24)与自然条件下形成的立枯物(CK)的差异;并采用分解袋法对此2种小叶章枯落物的早期分解过程进行了研究。结果表明,施氮处理下小叶章立枯物的氮、磷含量均高于CK,但碳含量显著降低(n=3,p<0.05)。施氮处理下形成的小叶章枯落物的分解速率明显大于CK,分解160d后两者的失重率分别为初始值的31.13%(N24)和28.50%(CK)。分解过程中,2种处理下枯落物有机碳的绝对含量都表现为净损失;其氮、磷浓度的变化趋势大致相同,分解160d后均发生了氮、磷的净释放,其中,N24的氮、磷净释放量分别是CK的64.91和2.11倍。外源氮输入影响下小叶章枯落物C/N和C/P值的降低,加快了该枯落物的分解速率,从而影响土壤有机质的累积和CO2的排放;而该枯落物分解过程中营养元素释放量的增大,还可能会影响土壤其它营养元素可利用性。  相似文献   

三种海洋浮游微藻的分子鉴定及培养条件研究     

徐娜  逄少军 《海洋科学》2012,36(10):13-25

采用分子生物学手段结合形态特征对海洋中生活着的浮游单细胞海藻进行准确无误的分类鉴定,正在成为一种普遍接受的方式。这样做的前提是首先需要从海水中分离得到纯藻株, 掌握它们的生活条件, 获得生物量, 并进而完成鉴定。作者对一株裸甲藻类似种和两株新分离的赤潮单细胞海藻进行了分离、培养和鉴定。核糖体18S RNA 基因(18S rDNA)和转录单元内间隔区(ITS)序列分析结果显示这三株藻分别为共生甲藻(Symbiodinium sp.)、锥状斯氏藻(Scrippsiella trochoidea)和赤潮异弯藻(Heterosigma akashiwo)。作者以广泛使用的光密度值A₆₈₀作为衡量藻体生物量变化的指标, 研究了这三种藻在不同培养条件下的生长。结果表明, 在批次培养中, 共生甲藻、锥状斯氏藻和赤潮异弯藻的生物量都在22℃达到最大, 分别为9.28×10⁵个/mL、7.9×10⁴个/mL 和4.75×10⁵个/mL; 强光和长时间光照促进生长, 但锥状斯氏藻在强光下更早进入衰退期; 三种藻都表现出较强耐受盐度变化的能力,在盐度为15～36 的培养液中生长良好; 营养实验表明, 使用882 μmol/L 的NO₃^- 和36.2 μmol/L 的PO₄^3-能够维持三种海藻的快速生长。  相似文献